Imaged with the same 20×/0.75 NA objective lens on a Yokogawa spinning-disk confocal equipped with a 512 × 512 pixel EM-CCD camera (inner square) and with the full FOV available on an Olympus FluoView 1000 CLSM (outer square; Tokyo, Japan). Scale bar, 100 μm.
FOV comparison for an SDCM and a CLSM ()