Darcy Wagner
Professor, Department of Biomedical Engineering
Professor, Department of Medicine, Division of Experimental Medicine
Canada Excellence Research ChairÌýin Lung Regenerative MedicineÌý
¹ó²¹³¦³Ü±ô³Ù²â,ÌýMeakins-Christie Laboratories ()
Scientist, Translational Research in Respiratory Diseases Program (RESP) at the Research Institute of the ÎÛÎÛ²ÝÝ®ÊÓƵ University Health Centre ()
Principal Investigator, ÎÛÎÛ²ÝÝ®ÊÓƵ Regenerative Medicine Network ()Ìý
Our lab focuses onÌýhow theÌýextracellular environmentÌýdirectsÌýstem cellÌýbehaviorÌýfor endogenous and exogenous lung tissue regeneration. We use a multidisciplinary approach using advances in materials science (polymer designÌýand synthesis), manufacturing (e.g.Ìý3D bioprinting), andÌýendogenous and iPSC-derived lung stem cellsÌýto construct these new models.Ìý
The main areas of interest of the lab are:Ìý
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Design and use of biologic (acellular) and synthetic scaffolds in diverse manufacturing approaches to bioengineer new lung tissue for transplantation.Ìý
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Understand the role of the extracellular matrix in directing lung stem cell behaviour in vivo and ex vivo during regeneration (normal and pathologic)ÌýÌý
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BuildÌýand validateÌýnew models of human lung tissueÌýand chronic lung diseaseÌýto reduce animal usage and for use as drug discovery and therapeutic screening platforms
°±ð²â·É´Ç°ù»å²õ:Ìýbioengineering, lung regeneration, biomaterials, stem cells, bioprinting, microfluidics, light sheet fluorescence microscopy, image analysis
Recent select publications:
Gvazava et al.ÌýLabel-Free High-Resolution Photothermal Optical Infrared Spectroscopy for Spatiotemporal Chemical Analysis in Fresh, Hydrated Living Tissues and Embryos. Journal of the American Chemical Society.Ìý2023, 145 (45): 24796–24808.
Ptasinski et al.ÌýModeling fibrotic alveolar transitional cells with pluripotent stem cell-derived alveolar organoids.ÌýLife Science Alliance. 2023, 6(8):e202201853.
Da Silva et al.ÌýFormalin-free fixation and xylene-free tissue processing preserves cell-hydrogel interactions for histological evaluation of 3D calcium alginate tissue engineered constructs.ÌýFront Biomaterial ScienceÌý2023, 2:1155919.
Alsafadi et al.ÌýSimultaneous isolation of proximal and distal lung progenitor cells from individual mice using a 3D printed guide reduces proximal cell contamination of distal lung epithelial cell isolations.ÌýStem Cell RepÌý2022, 17(12): 2718-2731. and
De Santis et al. Extracellular matrix reinforced bioinks for 3D bioprinting human tissue.ÌýAdv MaterialsÌý2021, 33(3):2005476.
Complete Publication List:
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