污污草莓视频

Imaging

污污草莓视频

NIKON CSU-X1 Spinning Disk Confocal

Applications: Intravital Imaging, 4D Confocal, Fast Kinetics, Widefield Fluorescence, Brightfield

Excitation:

- Four lasers lines for confocal imaging: 405nm, 488nm, 561nm, 640nm.
- Four LED lines for widefield fluorescence: 385nm, 488nm, 561nm, 621nm.
- Brightfield illumination.

Emission:

- Four single chanel emission filters: 455/50nm, 525/36nm, 605/52nm, 705/72nm.
- One quad chanels filter for high speed acquisition: DAPI/FITC/TRITC/CY5.

Detection:

- Confocal: Photometrics Prime BSI Express sCMOS Camera. 2048x2048, 95fps@11-bits/43fps@16-bits.
- Widefield and Brigthfield: Nikon Digital Sight 10, Color and Monochrome mode, 25 mm FOV, 23.9 megapixel.

Objectives:听10X/0.45 air, 20X/0.75 air, 16X/0.8 water dipping, 20X/1.0 water, 60X/1.42 oil.

Located in a biosafety level 2 room that include a BSC.

Image processing and analysis: Nikon imaging software (NIS) workstation and Bitplane Imaris 9.1.2

LEICA SP8 Laser Scanning Confocal and Multiphoton

Applications: 4D Confocal and Multiphoton Imaging, Spectral imaging, FLIM, FRET, FRAP modules, SHG

Excitation:

-听 Four solid state laser lines: 448 nm, 488 nm, 552 nm,
听 638 nm
-听 One tunable IR two-photons laser: 680-1080 nm

Detection:

-听 Two internal HyDs (high sensitivity detectors)
-听 Two internal PMTs
-听 One transmitted light PMT
-听 Two reflected light HyD
-听 One brightfield detector (Dodt gradient)

Objectives: 4x to 63x (including air, oil and multi-immersion)

Environmental control:听CO2, temperature, humidity.

Image processing and analysis: Leica LAS X听and Bitplane Imaris 9.1.2

听 听

Perfusion system for live tissue imaging
Perfusion system for live tissue imaging
听 听
Fly eye autofluorescence
Fly eye autofluorescence
Second Harmonic and two-photon fluorescence on human aorta
Second Harmonic and two-photon fluorescence on human aorta