Discover Our Research Initiatives听
Our lab adapts two state-of-the-art tools to perform high-throughput overexpression screens to look for novel players in human disease. The ORFeome library, constructed by Drs. David E. Hill, Marc Vidal, and Frederick P. Roth鈥檚 groups, is a collection of barcoded ORFs that are cloned into a Gateway compatible system. The newest version hOFReome 9.1听听includes ~90% of all human protein-coding genes, allowing for an unbiased proteome-wide screening for searching candidate biomarkers and therapeutic targets.
The second system, the Bxb-landing pad system听, was constructed by听Dr. Doug Fowler鈥檚 lab听听and improved by Dr. Frederick P. Roth鈥檚 group听to deliver exogenous DNA to a designated safe harbor locus in transfectable mammalian cell lines. Bxb landing pad system enables us to select gene-inserted cells with FACS for making every cell in the recombined pool express a single gene. Therefore, when we do an听en masse听transfection of our ORFeome collection, we are able to听express 鈥渙ne gene per cell鈥澨齱ith an artificial promoter to achieve 鈥渉omogenous gene expression鈥�.
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Principal Investigator听
Dr. Kim is currently pursuing the translation of functional genomics tools to precision medicine, by exploring novel approaches to evaluate proteome-scale gene overexpression effects under various conditions to find novel drug combinations to combat chemotherapeutic resistance. Furthermore, Dr. Kim's lab is expanding their interest to various biology such as extracellular vesicle biogenesis and delivery, regulation of transcription factor 鈥� promoter interaction, autophagy and apoptosis, immunotherapy, etc.听
Contributions to Science
- Development of an efficient sequence comparison tool, Word Conservation Probability听
- Systematic analysis of extracellular vesicles
- Construction of a web-based database for extracellular vesicles: EVpedia
- Proteome-wide screening of protein-protein interactions among human protein-coding genes
- Investigation of protein-protein interactions in various species
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